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Recent advances in chemiluminescent enzyme substrates Hashem Akhavan-Tafti* Lumigen Inc., 24485 W. 10 Mile Road, Southfield, MI 48034, USA      *(hat@lumigen.com) Lumigen has developed a family of chemiluminescent enzyme substrates based on the acridan ring nucleus which is now used worldwide in immunological and nucleic acid detection. A class of acridan esters and thioesters Lumigen™ PS produce bright chemiluminescence by reaction with HRP and peroxide. Lumigen PS-3 reagent provides extremely sensitive detection of HRP-conjugated proteins and nucleic acids in blotting assays. Recently we have developed a new class of compounds, Lumigen APS, having an acridan ring and an exocyclic enol phosphate group. Reaction of the Lumigen APS-5 substrate with AP and oxygen generates a transient dioxetanone intermediate which undergoes a chemiluminescent decomposition. Enhanced reagents incorporating lucigenin cause a constant chemiluminescence intensity to be reached in seconds at room temperature. Detection reagents for several other enzymes have been developed as well. We also report the development of chemiluminescent enzyme substrates based on other heterocyclic ring systems which collectively span the entire range of the visible spectrum. [Talk: akhavant.hashem.31011]

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