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Bioluminescent spectra in the presence of the dyes of xanthene series Marina A. Gerasimova*1; NS Kudryasheva2; AG Sizykh1 (1) Dep. of QE, Krasnoyarsk State University, Svobodny Ave., 79, Krasnoyarsk, 660041, Russia; (2) Institute of Biophysics SB RAN, Akademgorodok, Krasnoyarsk, 660036, Russia      *(marmot@geoserv.krasnoyarsk.su) The photophysical approach to study the mechanism of bioluminescence assumes research of energy transfer processes in the bioluminescent electron-excited systems. In the present work the mechanism of excitation energy transport was studied in the coupled enzyme system NADH: FMN-oxidoreductase - luciferase in the presence of series of xanthene dyes: fluorescein, eosin, erythrosin. The spectral overlap of bioluminescence and absorption spectra of the dyes determines the probability of the resonance energy transport and light absorption. The sensitized luminescence of dyes is a result of these processes. The spectra of the bioluminescence were recorded in the presence of the dyes of xanthene series. The intensity of sensitized fluorescence was compared with a spectral overlap and the heavy atom presence in the molecular structure of a dye. It was shown that external effect of heavy atom determines the intensity of sensitized fluorescence of dyes and bioluminescence quenching. These effects were connected with the intersystem crossing rate of the bioluminescent emitter and the dyes. It was found that the bioluminescent enzyme system is the hyperfine indicator of the xanthene dyes. [Poster: gerasimo.marina.65342]

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