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Intracellular views of bioluminescent systems in the genus Pyrocystis
Ruediger Hardeland*1; Gudrun Behrmann1; Walter Stickan2; Ina Siebert2
(1) Institute of Zoology and Anthropology, University of Goettingen, Berliner Str. 28, Goettingen, D-37073, Germany; (2) Institute for Scientific Film, Nonnenstieg 72, D-37075 Goettingen *(rhardel@gwdg.de)
In two differently organized Pyrocystis species, P. acuta and P. noctiluca, the intracellular distribution of bioluminescent microsources is shown by autofluorescence microscopy. In correspondence to its circadian rhythmicity, nocturnal luminescence is associated with retraction of chloroplasts from light emitting areas. In constant darkness, P. acuta exhibits circadian rhythms of spontaneous bioluminescence, whereas P. noctiluca emits very low amounts of light in the absence of mechanical or chemical stimuli and, therefore, shows only rhythms of stimulated luminescence. After triggering by citric acid, the intracellular spatio-temporal pattern of flashing is visualized in the nocturnal stage of P. noctiluca (1).
(1) Behrmann G, Hardeland R: Bioluminescence and diurnal rhythmicity in dinoflagellates. 1999; Videofilm C 2013 Inst. of Scientific Film Goettingen.[Talk: hardelan.ruedig.40941]
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