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Fluorescence analysis of reactive oxygen species by six isolates of A. fumigatus
U.-C. Hipler*1; U.Wollina1; D. Denning2; B.Hipler3
(1) Department of Dermatology, Friedrich-Schiller-University Jena, D-07743 Jena, Germany; (2) Department of Microbiology, University of Manchester, United Kingdom; (3) BMG LabTechnologies GmbH, Offenburg, Germany *(firstname.lastname@example.org)
Species of A.fumigatus are of interest in pathogenesis of dermatological diseases. The present study investigates whether Aspergillus species are capable to produce ROS by themselves.
The ROS generation of fungi has been investigated using the 2',7'-dichlorofluorescin-diacetate (DCFH-DA).Six isolates of A.fumigatus were investigated. These cell suspensions with concentrations of 105 to 107 cells/ mL were measured in the fluorometer FLUOstar (BMG, Offenburg, Germany).Detectable fluorescence was observed only at concentrations > 105/mL.The isolates AF 91 and AF 72 are resistant against itraconazole and AF 65 is amphotericin B resistant.
In conclusion, the morphological event of fungi, usually acknowledged as a major factor of virulence, is associated with significantly increased intracellular ROS formation.
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