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Mechanisms of lux genes expression in transgenic bacteria Elena E. Maksimova*; Popova Ludmila Yu. Institute of Biophysics SB RAS, Akademgorodok, Krasoyarsk 660036, Russia      *(lena@ibp.krasnoyarsk.su) Intentional or unintentional releases of transgenic microorganism (TM) into the environment require full knowledge of the fate of the introduced genetic elements. Simple method that give reproducible results needed to perform an appropriate risk assessment evaluation of the introduction of TM into the environment. Both the devising of a simple man-made microcosms and reliable sampling procedures are essential requirements to evaluate bacterial capability to survive. Yet, no study has investigated the efficiency of expression of cloned genes in the cells of transgenic bacteria subject for a long time to adverse environmental conditions. Expression of lux-genes was evaluated in the course of prolonged (540 days) introduction of E. coli Z905 strain into model aquatic microecosystems. The cells have been found to reduce their luminescence owing to: decrease of plasmid DNA concentration, changes in regulation of cloned genes, decrease of intracellular metabolism activity. The research carried out can promote employment of marker transgenic microorganisms in closed ecosystems of different purpose. [Poster: maksimov.elenae.67342]

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