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Too much of a good thing? Modulating the signal of acridinium-9-carboxamide salts. M Adamczyk; YY Chen; JR Fishpaugh; Phillip G. Mattingly*; JA Moore; Y Pan; K Shreder; Z Yu Abbott Laboratories, 100 Abbott Park Rd, D09NM, BLDG AP20, Abbott Park, IL, 60064-6016, USA      *(phillip.g.mattingly@abbott.com) A series of N10-(3-sulfopropyl)- N-sulfonylacridinium-9-carboxamide salts were synthesized in a three step sequence consisting of acylating a sulfonamide ester with acridine-9-carboxylic acid chloride, sulfopropylating the acridine N10 position with 1,3-propanesultone and finally removing the ester protecting group with aqueous HCl. The chemiluminescent profiles of the compounds in the series varied according to the steric demands of the sulfonamide substituents. The time required for the entire chemiluminescent signal to be harvested ranged from ten seconds to greater than one minute, yet the total light output remained essentially constant. There was a greater than 20 fold difference between the extremes of the series when the signal was acquired over a fixed interval (0-3 s.) Several compounds from the series were used to prepare tracers necessary to demonstrate the applicability of this signal modulation for use in chemiluminescent immunoassays. [Poster: mattingl.philli.49602]

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