Abstract Preview Page

This is an automatically generated preview of the abstract you selected.

Inhibition analysis of chicken heterophil luminol and lucigenin luminescence Gerald A. Merrill*1; RC Allen2 (1) Dept. of Clinical Investigation, Brooke Army Medical Center, Ft. Sam Houston, TX 78234-6200, USA; (2) Emory University, Atlanta, GA.      *(Gerald.Merrill@CEN.AMEDD.ARMY.MIL) Chicken heterophil leukocytes (CHL) have NADPH oxidase activity but lack myeloperoxidase (MPO). Stimulated CHL yields lucigenin chemiluminescence (CL) comparable to that of MPO-positive human neutrophil leukocytes (HNL), but CHL luminol CL is very small in comparison to HNL. Inhibitors are used to analyze CHL CL activities. Inhibition constants are calculated by Dixon analysis or reported as the concentration producing 50% inhibition. Azide and cyanide are potent inhibitors of luminol CL in HNL, but these MPO inhibitors do not inhibit luminol or lucigenin CL in CHL. Since these agents inhibit eosinophil peroxidase, lack of inhibition indicates that the small percentages of peroxidase-positive eosinophils in the CHL preparations are not responsible for the luminol CL observed. Iodoacetate and fluoride, pre-oxidase and pre-peroxidase inhibitors of glycolytic metabolism, effectively inhibit both lucigenin and luminol CL activities in CHL. Superoxide dismutase competitively inhibits both lucigenin and luminol CL in CHL, but catalase is ineffective as an inhibitor. Although luminol is efficiently dioxygenated by a MPO-dependent mechanism in HNL, this substrate does not exclusively measure peroxidase activity. [Poster: merrill.gerald.77392]

Return to the list of abstracts

E-mail   |  Conference Info  |   Index of Abstracts