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Study of the electronic excited states of bioluminescent emitter with dye molecules E. Nemtseva*1; N. Kudryasheva1; A.J.W.G.Visser2 (1) Institute of Biophysics, Krasnoyarsk, 660036, Russia; (2) MicroSpectroscopy Centre, Dept Biomolecular Sci., Wageningen Agricultural Univ., 6703 HA Wageningen, The Netherlands     *(lightbirdy@hotmail.com) The hypothesis on activity of upper electronic excited states of bacterial bioluminescent emitter has been verified with dye molecules as energy acceptors. Six compounds were selected having the energy of their fluorescent states in the range of 24700 to 32000 cm -1 (POPOP, MSB, anthracene, pyrene, 2-methoxy-naphtalene, p-terphenyl). The bacterial bioluminescent spectra of the coupled enzyme system NADH:FMN-oxidoreductase - luciferase in the presence of these compounds were studied. The weak sensitized fluorescence of the dyes, which have fluorescent states not higher than 26 000 cm-1, was registered. Since the energy of the fluorescent states of the dyes exceeds that of the bioluminescent emitter (21 700 cm-1) and their absorption spectra do not overlap with the bioluminescence spectrum, trivial light absorption and intermolecular resonance singlet-singlet energy transfer were excluded. The results obtained have confirmed the activity the energetic precursor in bacterial bioluminescence. Its energy can be localized as about 26000 cm-1. [Poster: nemtseva.e.13232]

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