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Visualization of bacteria in live animals using luciferase labeling Shahrokh Shabahang*; Aladar A. Szalay Dept. of Microbiology and Molecular Genetics, Dept. of Biochemistry, Loma Linda, CA. 92350, USA      *(sshabahang@som.llu.edu) Focal infection has been defined as a focus-induced irritation of the vegetative neurosystem causing neurovascular changes responsible for secondary pathology. Metastatic lesions arising from a focus of infection are currently linked to a number of significant systemic ailments including cardiac and autoimmune diseases. Examination of the evolution of these pathologic changes and formulation of therapeutic modalities necessitates the ability to visualize microorganisms in a dynamic system. Animal models have been used for detection and evaluation of the progression of metastatic tumors. In a recent study, Green Fluorescent Protein (GFP) was used for an in vivo investigation of metastatic tumors in whole mice. The authors suggested that GFP systems are advantageous over luciferase systems because they do not require exogenous substrate delivery. In this presentation, we will demonstrate visualization of bacteria in whole live animals using the luciferase system. Visualization of bacterial cells will be shown in various tissues including organs colonized following intra-venous injections of the labeled cells. [Talk: shabahan.shahro.47001]

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