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Bioluminescent assay of ATP leakage from yeast cells after their photodynamic damage Natalia Ugarova*1; NA Romanova1; EV Pometun1; AP Savitsky1; LYu Brovko2 (1) Chemistry Department, Lomonosov Moscow State University, Moscow, 119899, Russia; (2) Canadian Research Institute for Food Safety, University of Guelph, Guelph, Ontario NIG 2W1, Canada      *(unn@enzyme.chem.msu.ru) Phodynamic destruction of cells by a photosensitizer selectively accumulated in the cell wall and activated by visible light was used to release intracellular ATP that was then measured by bioluminescent method. The illumination of yeast cells in the presence of photosensitizers, derivatives of certain hematoporphyrins, with red light resulted in cell wall damage and leakage of intracellular ATP. The same illumination of non-treated cells had no effect on yeast cells. The extent of cell damage depended on surface concentration of photosensitizer, dose and power of illumination. Up to 50% of intracellular ATP content was released upon short time ( up to 5 min) illumination. The quantity of ATP released was proportional to the number of cells in the sample. [Poster: ugarova.natali.49482]

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