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Inducible gene expression in vivo using a Renilla luciferase-GFP fusion construct Yong A. Yu*1; Aladar Szalay2 (1) Dept. of Biochemistry; Dept. of Human Anatomy, Loma Linda University, Loma Linda, CA 92350, USA; (2) Department of Biochemistry, Loma Linda University, Loma Linda, CA 92350      *(chestnut9@hotmail.com) We use a steroid induced gene expression system as a molecular switch to study the regulatable transgene expression in vivo. Monitoring of the gene expression from this inducible system is facilitated through the use of a low-light video camera and UV microscope to detect the transferred marker gene (Renilla luciferase - GFP fusion) expression. We show significant induction of both luciferase and GFP expression in transiently tranfected cells upon the treatment with the inducer drug. This induction system was also confirmed in nude mice which received cellular transplants using the ex vivo-modified cells. Significant transgene expression in the cellular implants was shown in the animals when the inducer drug was delivered to the animals by intraperitoneal injection. The establishment of the non-invasive external monitoring method for observing inducible gene expression in live animals will help us to address fundamental biological and therapeutical questions by analyzing gene functions at specific stage of animal development. For example, study the HLA-G gene activation and protection of implants from natural killer cells. The relevance of this technology for gene therapy will also be addressed. [Talk: yu.yonga.95881]

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