People

Seng Hui Low, Ph.D.

Adjunct Associate Professor

Contact Information

Phone: (805) 893-4725
Fax: (805) 893-4724
E-mail: shlow@lifesci.ucsb.edu

Office: LSB - Room 2111

Molecular, Cellular, and Developmental Biology
University of California, Santa Barbara
Santa Barbara, CA 93106-9610

Area of general research interest:

The role of membrane fusion and SNAREs during mammalian cell cytokinesis; targeting of syntaxins in polarized epithelial cells; polycystin-1 interacting proteins.

Professional education and positions held:

2005-present	Adjunct Associate Professor, Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara
2005-present	Project Scientist, Neuroscience Research Institute, UCSB
2004-2005	Assistant Professor, Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University
1999-2005	Project Staff, Department of Cell Biology, The Cleveland Clinic
1994-1999	Postdoctoral Researcher in the Department of Anatomy, School of Medicine, University of California at San Francisco, in the lab of Prof. Keith E. Mostov
1990-1994	Ph. D. Program, Institute of Molecular and Cell Biology, National University of Singapore, Prof. Wanjin Hong, Advisor
1987-1989	Master of Science, Department of Microbiology, National University of Singapore
1985-1986	Honours Program, Department of Microbiology, National University of Singapore
1982-1985	Undergraduate studies, National University of Singapore

Awards and Fellowships

- Scientist Development Grant, American Heart Association, 2002-2006
- Irvington Institute for Immunological Research, Postdoctoral Fellowship, 1995-1997
- Human Frontier Science Program, Short-Term Fellowship, 1995
- National University of Singapore Postgraduate Scholarship, 1987-1989

Memberships in professional societies

American Society for Cell Biology

Publications

Kidd GJ, Yadav VK, Huang P, Brand SL, Low SH, Weimbs T, Trapp BD (2006)
A dual tyrosine-leucine motif mediates myelin protein P(0) targeting in MDCK cells.
Glia Jun 20;. [Epub ahead of print]

Sharma N, Low SH, Misra S, Pallavi B, Weimbs T (2006)
Apical targeting of syntaxin 3 is essential for epithelial cell polarity
The Journal of Cell Biology 173, 937–948

Shillingford JM, Murcia NS, Larson CH, Low SH, Hedgepeth R, Brown N, Flask CA, Novick AC, Goldfarb DA, Kramer-Zucker A, Walz G, Piontek KB, Germino GG, Weimbs T (2006)
The mTOR pathway is regulated by polycystin-1 and its inhibition reverses renal cystogenesis in polycystic kidney disease.
Proceedings of the National Academy of Sciences of the USA 103, 5466-5471

Low SH, Vasanji A, Nanduri J, He M, Sharma N, Koo M, Drazba J, Weimbs T. (2006)
Syntaxins 3 and 4 Are Concentrated in Separate Clusters on the Plasma Membrane prior to the Establishment of Cell Polarity.
Molecular Biology of the Cell 17, 977-989

Low SH, Vasanth S, Larson CH, Mukherjee S, Sharma N, Kinter MT, Kane ME, Obara T, and Weimbs T (2006)
Polycystin-1, STAT6 and P100 function in a novel pathway that transduces ciliary mechanosensation to gene expression and is activated in polycystic kidney disease.
Developmental Cell 10, 57-69

Low, S.H., Li, X., Miura, M., Kudo, N., Quinones, B. and Weimbs, T. (2003) Syntaxin 2 and Endobrevin are Required the Terminal Step of Cytokinesis. Developmental Cell 4, 753-759

Weimbs, T., Low, S.H., Li, X. and Kreitzer, G. (2003) SNAREs and epithelial cells. Methods 30, 191-197

Kreitzer, G., Schmoranzer, J., Low, S.H., Li, X., Gan, Y., Weimbs, T., Simon, S.M. and Rodriguez-Boulan, E. (2003). Three-dimensional Analysis of Post-Golgi Carrier Exocytosis in Epithelial Cells. Nature Cell Biology 5, 126-136

Sherief, M.H., Low, S.H., Miura, M., Kudo, N., Novick, A.C. and Weimbs, T. (2003). Matrix Metalloproteinase Activity in Urine of Renal Cell Carcinoma Patients Leads to Degradation of Extracellular Matrix Proteins: Possible Use as a Screening Assay. J. Urology 169, 1530–1534

Low, S.H., Marmorstein, L.Y, Miura, M., Li, X., Kudo, N., Marmorstein, A.D. and Weimbs, T. (2002). Retinal pigment epithelial cells exhibit unique expression and localization of plasma membrane syntaxins which may contribute to their trafficking phenotype. Journal of Cell Science 115, 4545-4553

Li X, Low S.H., Miura M, and Weimbs T (2002) Differential SNARE expression and localization in renal epithelial cells suggests mechanism for establishment of distinct trafficking phenotypes. Am J Physiol 283: F1111–F1122.

Lim, S.-N., Bonzelius, F., Low, S.H., Wille, H., Weimbs, T., and Herman, G.A. (2001) Identification of a novel class of small endosome-derived vesicles as a major cellular pool for recycling proteins. Mol. Biol. Cell, 12, 981-995

Low, S. H., Miura, M., Roche, P. A., Valdez, A. C., Mostov, K. E., and Weimbs, T. (2000). Intracellular re-direction of plasma membrane trafficking after loss of epithelial cell polarity. Mol. Biol. Cell 11, 3045-3060

Low, S. H., Chapin, S. J., Wimmer, C., Whiteheart, S. W., Kömüves, L. K., Mostov, K. E., and Weimbs, T. (1998). The SNARE machinery is involved in apical plasma membrane trafficking in MDCK cells. J Cell Biol. 141, 1503-1513

Low, S.H., Roche, P.A., Anderson, H.A., van Ijzendoorn, S.C.D., Zhang, M., Mostov, K.E., and Weimbs, T. (1998) Targeting of SNAP-23 and SNAP-25 in polarized epithelial cells. J. Biol. Chem. 273, 3422-3430

Weimbs, T., Low, S. H., Chapin, S. J., Mostov, K. E., Bucher, P. and Hofmann K. (1997). A conserved domain is present in different families of vesicular fusion proteins: A new superfamily. Proc. Natl. Acad. Sci. USA 94, 3046-3051

Weimbs, T., Low, S. H., Chapin, S.J., and Mostov, K.E. (1997). Apical targeting in polarized epithelial cells: there's more afloat than rafts. Trends in Cell Biology. 7, 393-399

Low, S. H., Chapin, S. J., Weimbs, T., Komuves, L. G., Bennett, M. K., and Mostov, K. E. (1996). Differential localization of syntaxin isoforms in polarized Madin-Darby canine kidney cells. Mol. Biol. Cell. 7, 2007-2018.

Mostov, K.E., Altschuler, Y., Chapin, S.J., Enrich, C., Low, S.H., Luton, F., Richman-Eisenstat, J., Singer, K.L., Tang, K., Weimbs, T. (1995). Regulation of Protein Traffic in Polarized Epithelial Cells: The Polymeric Immunoglobulin Receptor Model. Cold Spring Harbor Symposium of Quantitative Biology 60, 775-781

Tang, B.L., Low, S.H., Wong, S.H., and Hong, W. (1995) Cell type differences in Golgi retention signals for transmembrane proteins. Eur. J. Cell Biol. 66, 365-374.

Low, S. H., Tang, B. L., Wong, S. H., and Hong, W. (1995) Retardation of a surface protein chimera at the cis Golgi. Biochemistry. 34, 5618-5626.

Tang, B.L., Wong, S.H., Low, S.H., Subramaniam, V.N., and Hong, W. (1994) Cytosolic factors block antibody binding to the C-terminal cytoplasmic tail of the KDEL receptor. Eur. J. Cell Biol. 65, 298-304.

Low, S.H., and Hong, W. (1994) Protein trafficking in and out of the Golgi apparatus. Trends in Glycoscience and Glycotechnology 6, 310-327.

Low, S. H., Tang, B. L., Wong, S. H., and Hong, W. (1994) Golgi retardation in Madin-Darby canine kidney and Chinese hamster ovary cells of a transmembrane chimera of two surface proteins. J. Biol. Chem. 269, 1985-1994.

Low, S.H., Wong, S.H., Tang, B.L., and Hong, W. (1994) Effects of NH4Cl and nocodazole on polarized fibronectin secretion vary among different epithelial cell types. Mol. Membr. Biol. 11, 45-54.

Tang, B.L., Wong, S.H., Qi, X.L., Low, S.H., and Hong, W. (1993) Molecular cloning, characterization, subcellular localization and dynamics of p23, the mammalian KDEL receptor. J. Cell Biol. 120, 325-338.

Tang, B.L., Wong, S.H., Low, S.H., and Hong W. (1992) The transmembrane domain of N-glucosaminyltransferase I contains a Golgi retention signal. J. Biol. Chem. 267, 10122-10126.

Low, S.H., Tang, B.L., Wong, S.H., and Hong, W. (1992) Selective inhibition of protein targeting to the apical domain of MDCK cells by brefeldin A. J. Cell Biol. 118, 51-62.

Wong, S.H., Low, S.H., and Hong, W. (1992) The 17-residue transmembrane domain of b-galactoside a-2,6-sialyltransferase is sufficient for Golgi retention. J. Cell Biol 117, 245-258.

Low, S.H., Wong, S.H., Tang, B.L., Tan, P., Subramanium, V.N., and Hong, W. (1991) Inhibition by brefeldin-A of protein secretion from the apical cell surface of Madin-Darby canine kidney cells. J. Biol. Chem. 266, 17729-17732.

Low, S.H., Wong, S.H., Tang, B.L., and Hong, W. (1991) Involvement of both vectorial and transcytotic pathways in the preferential apical cell surface localization of rat dipeptidyl peptidase IV in transfected LLC-PK1 cells. J. Biol. Chem. 266, 19710-19716.

Low, S.H., Wong, S.H., Tang, B.L., Subramanium, V.N., and Hong, W. (1991) Apical cell surface expression of rat dipeptidyl peptidase IV in transfected Madin-Darby canine kidney cells. J. Biol. Chem. 266, 13391-13396.

Low, S. H., Thong, T. W., Ho, T. H., Lee, Y. S., Morita, T., Singh, M., Yap, E. H., and Chan, Y. C. (1990) Prevalence of human papillomavirus types 16 and 18 in cervical carcinomas: a study by dot and Southern blot hybridization and the polymerase chain reaction. Jpn. J. Cancer Res. 81, 1118-1123.