Nearest-neighbor dendrogram showing the relationship of all SNARE proteins among each other. The alignment was used as a basis for this dendrogram analysis. Five SNARE families can be identified by their clustering: the synaptobrevin/VAMP, Bos1, SNAP-25 (N- and C-terminal), syntaxin and Bet1 families. Syntaxin 6 may form another subfamily together with the proteins TSL-2 and TSL-3 that have been identified from EST databases but are so far uncharacterized*. We have identified a new mammalian member of the Bos1 family: EST AA616379. Stx = syntaxin. TSL = t-SNARE like protein*. Two-letter species abbreviations after the protein name: RN, rattus norvegicus; HS, homo sapiens; CE, caenorhabditis elegans; SC, saccharomyces cerevisiae; AT, arabidopsis thaliana; MM, mus musculus; CG, cricetulus griseus. NT and CT refers to the N-terminal and C-terminal coiled-coil domain of SNAP-25-related proteins, respectively. Bifurcation points confirmed in 80-100 bootstrap replicates (out of 100) are marked by filled triangles, in 50-80 replicates by filled circles, in 20-50 replicates by open circles and in less than 20 replicates by no label.

*see the following reference: Weimbs, T., Low, S. H., Chapin, S. J., Mostov, K. E., Bucher, P., and Hofmann, K. (1997). A conserved domain is present in different families of vesicular fusion proteins: A new superfamily. Proc. Natl. Acad. Sci. USA 94, 3046-3051

For questions/comments etc. contact Thomas Weimbs at weimbs@lifesci.ucsb.edu