BACKGROUND INFORMATION AND LITERATURE REFERENCES

Contents:

1. Background information:
   RAPDs (Randomly amplified polymorphic DNA).
   Fundamentals of Tetrahymena Genetics
   MIC linkage and chromosome mapping
   MAC Genetics

2. Key references:
   RAPD DNA polymorphisms
   Mapping RAPDs in Tetrahymena: MIC and MAC
   Previous Tetrahymena maps
   Reviews

3. Other references cited

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1. Background information

RAPDs (Randomly amplified polymorphic DNA).
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Fundamentals of Tetrahymena Genetics
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MIC linkage and chromosome mapping
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MAC Genetics: DNA rearrangements during MAC differentiation, 
assortment, coassortment groups, MIC-limited sequences.
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2. Key references

RAPD DNA polymorphisms
- Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A. and Tingey S.V. 
(1990): DNA polymorphisms amplified by arbitrary primers are useful genetic 
markers. Nucl. Acids Res., 18: 6531-6535. 

Application of the RAPD method to mapping the Tetrahymena micro- and macro-
nucleus, respectively:
- Brickner J.H., Lynch T.J., Zeilinger D. & Orias E. (1996) Identification, 
mapping and linkage analysis of randomly amplified DNA polymorphisms in 
Tetrahymena thermophila. Genetics, 141: 1315-1325.
- Longcor M.A., Wickert S.A., Chau M.-F. & Orias E. (1996) Coassortment of 
genetic loci during macronuclear division in Tetrahymena thermophila. Eur. 
J. Protistol., in press (Oct. 1996 issue).

Previously published Tetrahymena maps
- Bruns P.J. and Cassidy-Hanley D. (1993): Tetrahymena thermophila. In: 
O'Brien S.J. (ed.): Genetic Maps: Locus Maps of Complex Genomes, 6th ed., 
pp. 2.175-2.179. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, 
N.Y. 

Reviews
- Bruns P.J. (1986): Genetic organization of Tetrahymena. In: Gall J.G. 
(ed.):  Molecular Biology of the Ciliated Protozoa, pp. 27-44.  Academic 
Press NY. 
- Orias E. (1986): Ciliate Conjugation. In: Gall J.G. (ed.):  Molecular 
Biology of the Ciliated Protozoa, pp. 45-84. Academic Press NY. 
- Orias E. and Bruns P.J. (1975): Induction and isolation of mutants in 
Tetrahymena. In: Prescott D.M. (ed.): Methods in Cell Biology, vol. 13, 247-
282. Academic Press, NY. 
- Yao M.-C. (1989): Site-specific chromosome breakage and DNA deletion in 
ciliates. In Mobile DNA (Berg D.E. & Howe M.M., eds.), American Society for 
Microbiologists, Washington DC, 715-734. 

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3. Other references cited
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