GFP-linked cDNA sequences encoding peptides with putative cortical localizations

Summary table

Cell line #

Localization

Insert

length

% GC

Blast-X matches

18-3B2

23-19C11

Microtubule-associated

ditto

607

 

30

Protein kinases (weak but consistent,

confined to one region of the sequence)

 

19-6E9

Ciliary-row associated

587

32

NMDA-receptor associated protein

(highly significant; ~30% amino-acid identity in large portion of the sequence)

19-12G4

Oral apparatus; tetrin-like

678

37

No significant matches

21-24G6

24-2H9

29-6F5

29-22C4

Epiplasmic

ditto

ditto

ditto

463

44

25kDa Ca2+ binding protein

(99% identity)

25-11H4

Oral apparatus; tetrin like

(high background)

308

44

ATP synthase—a chain

(70-80% amino acid identity throughout)

27-8D5

Oral apparatus; tetrin like

341

34

No significant matches

Stop codon at bases 289-292

 

References

Tetrin: Honts & Williams (1990). J. Cell Sci. 96, 293-302; Dress et al. (1992) J. Struct. Biol. 108, 187-194.

25 kDa Ca2+ binding protein: Takemasa et al. (1989) J. Biol. Chem. 264, 19293-19301; Hanyu et al., Exp. Cell Res. 219, 487-493.

NMDA-receptor-associated protein: Michaelis (1998) Progr. Neurobiology 54, 369-915 (see Section 3.8.2., pp. 388-389); Schweitzer et al. (1998) Molec. Cell. Neuroscience 11, 260-273.

 



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