GFP-linked cDNA sequences encoding peptides with putative cortical localizations
Summary table
Cell line # |
Localization |
Insert length |
% GC |
Blast-X matches |
18-3B2 23-19C11 |
Microtubule-associated ditto |
607
|
30 |
Protein kinases (weak but consistent, confined to one region of the sequence)
|
19-6E9 |
Ciliary-row associated |
587 |
32 |
NMDA-receptor associated protein (highly significant; ~30% amino-acid identity in large portion of the sequence) |
19-12G4 |
Oral apparatus; tetrin-like |
678 |
37 |
No significant matches |
21-24G6 24-2H9 29-6F5 29-22C4 |
Epiplasmic ditto ditto ditto |
463 |
44 |
25kDa Ca2+ binding protein (99% identity) |
25-11H4 |
Oral apparatus; tetrin like (high background) |
308 |
44 |
ATP synthase—a chain (70-80% amino acid identity throughout) |
27-8D5 |
Oral apparatus; tetrin like |
341 |
34 |
No significant matches Stop codon at bases 289-292 |
References
Tetrin: Honts & Williams (1990). J. Cell Sci. 96, 293-302; Dress et al. (1992) J. Struct. Biol. 108, 187-194.
25 kDa Ca2+ binding protein: Takemasa et al. (1989) J. Biol. Chem. 264, 19293-19301; Hanyu et al., Exp. Cell Res. 219, 487-493.
NMDA-receptor-associated protein: Michaelis (1998) Progr. Neurobiology 54, 369-915 (see Section 3.8.2., pp. 388-389); Schweitzer et al. (1998) Molec. Cell. Neuroscience 11, 260-273.