BLAST X analysis of randomly selected Tetrahymena cDNA clones
The Turkewitz lab made the cDNA library, Ron Pearlman's group obtained the cDNA sequences,
and Ron Pearlman did the preliminary BLAST analysis reported below.
Clone Fragment* BLAST X matches Reading frame # size (Kb) -------------------------------------------------------------------- 4 0.9 60S ribosomal -3 & -1 protein-L19 frameshift 5 1.4 glucokinase +3 6 1.3 glycerophosphoryl diester +2 phosphate 8 0.3 GRL 1p-Tetrahymena* -3 11 0.6 ? 17 1.2 60S ribosomal protein L10 +2 20 1.0 N-methyl-D-aspartate receptor +3 associated protein 22 0.9 ? 23 0.8 ? 26 0.6 60S ribosomal protein L37A +3 27 1.3 ? 28 1.4 S-adenosylhomocysteinase +2 30 1.1 40S ribosomal protein S27 +1 31 <vector cathepsin L-Paramecium- +3 (cysteine protease) 34 0.6 ? 35 0.8 + 0.6 (?) ? 37 ? ? 38 0.5 NADH dehydrogenase/cyt B (?) -2 39 ? Ribosomal protein L23(17A) +1 40 0.7 hypothetical prot (ND repeat?) +1 41 0.5 Ribosomal protein S8 +2 42 0.7 Possible weak (?) +2 43 1.2 Possible plasminogen & others -3 45 0.6 ? 46 1.5 possible mammalian channel receptor -3 48 0.7 Ribosomal protein L35 +2R;-3F 49 ? ? 50 0.7 ? 52 1.6 GRL 3p-Tetrahymena -2 53 0.6 H4 tetrahymena-P02311 +2R;-3F 60 ? long polyA-short sequence-BLAST n 100% match but not informative 61 0.5 60S Ribosomal protein L22 +1 65 1.2 ? 69 0.8 GRL 7P & initiation and co- stimulation Factor-Tetrahymena 70 0.5 ?-short BLAST n-uninformative 76 0.6 ovomucoid serine protease +3 inhibitor PSTI type 79 0.5 + 1.0 (?) Ribosomal protein S8 -1 81 0.5 + 0.8 (?) ? 83 0.5 possible-weak +3 --------------------------------------------------------------------
* BstX1
fragment; ?: no obvious hit but needs further analysis.
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