Chapter Titles
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Authors
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Laboratory and Evolutionary
History of Tetrahymena
thermophila
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D. L. Nanney and E. M. Simon,
University of Illinois in Urbana.
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Cell Biology of Tetrahymena thermophila
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J. Frankel, University of Iowa.
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Tetrahymena Genetics: Two Nuclei
are Better Than One
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K. M. Karrer, Marquette University.
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Tetrahymena as a Laboratory
Organism: Useful Strains, Cell Culture, and Cell Line Maintenance
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E. Orias, E. P. Hamilton, and
J. D. Orias, University of California at Santa Barbara.
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Long-term Storage
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P. Bruns, H. R. Smith, and D.
Cassidy-Hanley, Cornell University.
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Genetic Crosses: Setting up
Crosses, Testing Progeny, and Isolating Phenotypic Assortants
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E. P. Hamilton and E. Orias,
University of California at Santa Barbara.
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Methods for Genetic Analysis
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P. Bruns and D. Cassidy-Hanley,
Cornell University.
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Isolation of Micronuclear and
Macronuclear DNA
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S. L. Allen, University of Michigan.
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Genetically Sorting a Collection
of Tetrahymena Mutants
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E. Orias and E. P. Hamilton,
University of California at Santa Barbara.
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Genetically Mapping New Mutants
and Cloned Genes
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E. P. Hamilton and E. Orias,
University of California at Santa Barbara.
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Selection of Motility Mutants
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D. G. Pennock, Miami University
of Ohio.
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Nuclear and Cytoskeletal Fluorescence
Microscopy Techniques
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E. S. Cole and K. R. Stuart,
St. Olaf College.
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Nuclear and Cortical Histology
for Bright Field Microscopy
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E. S. Cole and K. R. Stuart,
St. Olaf College
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Fixation of Tetrahymena Cells
for Electron Microscopy
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W. Dentler, University of Kansas.
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Immuno-electron Microscopy of
Tetrahymena
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R. H. Gavin, J. G. Hoey, and
J. A. Garces, Brooklyn College
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Regulated Protein Secretion
in Tetrahymena
thermophila
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A. P. Turkewitz, N. D. Chilcoat,
A. Haddad, and J. W. Verbsky, University of Chicago.
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Electrophysiology of Tetrahymena
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T. M. Hennessey and H. G. Kuruvilla,
State University of New York in Buffalo.
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Isolation and Characterization
of in vivo Modified Histones and Activity Gel Assay for Identification of Histone Acetyltransferase
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E. A. Wiley, C. A. Mizzen, and
C. D. Allis, University of Virginia.
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Tetrahymena Telomerase Activity,
Purification, and Reconstruction
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C. Autexier, McGill University.
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Study the Telomerase RNA in
Tetrahymena
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E. Blackburn, D. Gilley, T.
Ware, A. Bhattacharyya, K. Kirk, and H. Wang, University of California in San Francisco.
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Isolation and Characterization
of 22S Outer Arm Dynein from Tetrahymena Cilia
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T. M. Gibson and D. J. Asai,
Purdue University.
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Preparation of Cytoskeletal
Fractions from Tetrahymena
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N. E. Williams, University of
Iowa.
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Immunoprecipitation Procedures
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N. E. Williams, University of
Iowa.
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Tetrahymena Calcium-binding
Proteins, TCBP-25 and TCBP-23
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O. Numata, K. Hanyu, T. Takeda,
and Y. Watanabe, University of Tsukuba.
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Microinjection of Tetrahymena thermophila
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M. -C. Yao, D. L. Chalker, C.
Randolph, and J. G. Ward, Fred Hutchinson Cancer Research Center.
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Transient and Stable DNA Transformation
of Tetrahymena
thermophila by Electroporation
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J. Gaertig and G. Kapler, University
of Georgia and Texas A&M University.
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Biolistic Transformation of
Macro- and Micronuclei
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P. Bruns and D. Cassidy-Hanley,
Cornell University.
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Knockout Heterokaryons Enable
Facile Mutagenic Analysis of Essential Genes in Tetrahymena
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M. A. Gorovsky, B. Hai, and
J. Gaertig, University of Rochester
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Creation and Use of Antisense
Ribosomes in Tetrahymena
thermophila
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M. -C. Yao, Q. Fan, and R. Sweeney,
Fred Hutchinson Cancer Research Center.
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Protein Tagging in Tetrahymena
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M. A. Gorovsky and L. Yu, University
of Rochester.
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Appendix
I: Genetic Nomenclature Rules for Tetrahymena
thermophila
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S. L. Allen, University of Michigan.
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Appendix II: Codon Usage in
Tetrahymena
thermophila
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J. Wuitschick and K. Karrer,
Marquette University.
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