List of genes and chromosome breakage sites
Contents:
Chromosome breakage site polymorphisms (CBSP) - Numerically ordered list
Conventional genes - Alphabetical list
Cloned-gene RFLPs - Alphabetical list
Chromosome breakage site polymorphisms (CBSP) - Numerically ordered list
Key to the list of CBSP and abbreviations used:
"Cbs Locus " column. "A" and "T" suffixes refer to orientation with respect to Cbs site (see note
to Diagram B of the Illustrated Guide).
"Linkage group (lg)" column: See under RAPD key and abbreviations.
"Coassortment group (cag)" column:
- MiLtd: MIC-limited segment.
- Other abbreviations as in RAPD key and abbreviations.
"Primers" column: See primer sequences in the PCR primers page.
"Tr" column: PCR reannealing temperature.
"Size (Kb)" column. >B or <B indicates that the C3 band is slightly larger or smaller than the B band, respectively.
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|
Cbs
|
PCR Primers |
Tr |
Size
(Kb) |
Linkage
|
Coassortment |
Comments |
|
Locus
|
1 2 |
oC |
B
C3 |
group
(MIC) |
group (MAC) |
|
|
|
|
|
|
|
|
|
|
Cbs1
|
Cbs1A
Cbs1T |
|
|
lg5X-JB04
|
MIC-Limited |
2
Cbs in B; 1 in C3 |
|
Cbs4
|
Cbs4A
Cbs4T |
|
|
lg5X-JP02
|
MIC-Limited |
|
|
Cbs826
|
rTt826
ACbs |
|
0.24
>B |
lg1R-GAL1
|
MIC-Limited
|
|
|
Cbs826A
|
rTt826+35
Tel14 |
|
|
lg1R-GAL1
|
cag1R-Cbs826A |
|
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Note: Depending on how the
Cbs polymorphism is tested, it can behave as MIC-limited or MAC-destined (see Cbs explanatory diagram).
Conventional genes - Alphabetical list
Key to the list of conventional genes and abbreviations used:
"Linkage group (lg)" and "Coassortment group (cag)" columns: See under RAPD key and abbreviations.
"Phenotype test" column.
- GE: gel electrophoresis.
- ug/ml: drug concentration required to test the phenotype
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|
|
Locus
|
Phenotype
test |
Linkage
|
Coassortment |
Gene
name & Comments |
|
name |
|
Group
(MIC) |
group (MAC) |
|
|
|
|
|
|
|
|
AcpA
|
Starch
GE |
lg3R-AcpA
|
ND |
Acid
Phosphatase 1 |
|
CHX1
|
15
ug/ul |
lg1R-CHX1
|
cag1R-CHX1
|
Cycloheximide
resistance |
|
EstA
|
Starch
GE |
lg1R-CHX1
|
cag1R-EstA
|
Esterase
1
|
|
GAL1
|
250
ug/ul |
lg1R-GAL1
|
cag1R-GAL1
|
2-deoxy-D-galactose
resistance |
|
mat
|
MT
spectrum |
lg1L-mat
|
cag1L-mat
|
Mating
type (MT) |
|
MPR1
|
30
ug/ul |
lg2R-MPR1
|
cag2R-MPR1
|
6-methylpurine
resistance |
|
PMR1
|
100
ug/ul |
lg1L-mat
|
cag1L-rDNA
- |
Paromomycin
resistance |
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Note that many more conventional genes have been assigned to chromosome arms (Bruns, Hanley-Cassidy
and Merriam, personal communication).
Cloned-gene RFLPs - Alphabetical list
Key to the list of cloned-gene RFLPs and abbreviations used:
"Linkage group (lg)" and "Coassortment group (cag)" columns: See under RAPD key and abbreviations.
"Restriction enzyme" column: Enzyme used to reveal and map the polymorphism.
"B band size (Kb)" column: Size of a (the) polymorphic band in inbred strain B DNA.
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|
Locus
|
Restriction
|
B
band |
Linkage
|
Coassortment |
Gene
name & Comments |
|
Name |
Enzyme
|
size
(Kb) |
group
(MIC) |
group (MAC) |
|
|
|
|
|
|
|
|
|
FBR1
|
|
|
lg3L-FBR1
|
cag3L-FBR1
|
Fibrillarin
|
|
GRL3
|
|
|
lg1R-GAL1
|
cag1R-GRL3
|
Granular
Lattice Protein 3 |
|
GRL4
|
|
|
lg1R-GAL1
|
cag1R-GRL3
|
Granular
Lattice Protein 4 |
|
GRL7
|
|
|
lg1R-GAL1
|
cag1R-GRL7
|
Granular
lattice protein 7 |
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