Catalog of Tetrahymena thermophila loci tested for linkage
Updated 12-16-99
This
page contains links to technical specifications or phenotypes as well as MIC & MAC map locations associated
with each mapped locus.
Related resources in
this web site:
-
Hierarchically-organized summary of MIC and MAC map locations and
-
Illustrated guide to the relationship between genetic views of the MIC and MAC genomes: linkage and coassortment groups.
Types of loci linked
to on this page:
-
RAPD DNA polymorphisms. More than 350 loci are listed; they constitute the framework of our genetic map. A key to the RAPD
polymorphisms and abbreviations used is included.
-
Chromosome breakage site polymorphisms (CBSP). These are B-C3 polymorphisms associated with particular chromosome breakage sites, and they serve
to anchor the physical map to the genetic map. The cloning and identification of Cbs and associated polymorphisms
around the genome is actively in progress as a collaboration of the Bruns and Orias labs.
- Conventional genes.
This list includes genes that happened to be segregating in the panels used to map RAPDs or in ad hoc panels constructed
for special purposes to map other mutants. Current methods for mapping mutant genes are described in a chapter
by Hamilton and Orias, in the Asai and Forney in the Tetrahymena volume of Methods in Cell Biology. The linkage
mapping of mutant genes will be expedited by the development of universal tester panels (e.g., for deletion mapping),
recently started as a collaboration of the Bruns and Orias labs.
- Cloned-gene RFLPs. This list includes RFLPs mapped as a demonstration project or for other special purposes. Note that
the eventual availability of the Tetrahymena genomic sequence will obviate a genomic-scale effort to map cloned
genes through associated RFLPs.
Loci:
Other pages:
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